کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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3392761 | 1221292 | 2015 | 5 صفحه PDF | دانلود رایگان |
SummaryBackgroundThe diagnosis of acute malaria in non-endemic countries is still carried out largely by microscopic examination of thick and thin smears or rapid diagnostic tests. Low-density infections might be missed, however, but the more sensitive PCR is more expensive, complex and requires considerable more time.MethodWe examined the suitability of a new loop-mediated isothermal DNA-amplification kit (LAMP) for malaria diagnosis in febrile returning travellers in comparison to qPCR and microscopic examination in a prospective study in a non-endemic setting at the Swiss TPH.ResultsAmong 205 complete datasets, 43 samples were positive for malaria by microscopy, with Plasmodium falciparum (35 cases) being the most frequent species. All these samples were positive by both LAMP and qPCR, too. An additional 4 samples negative by microscopy were positive by both LAMP and qPCR. Three of these samples were follow-up samples taken after start of treatment in patients originally identified as positive by microscopy.ConclusionsThe LAMP performed exactly as did the qPCR and is a very valuable diagnostic alternative with a potential of being used also in endemic settings.
Journal: Travel Medicine and Infectious Disease - Volume 13, Issue 2, March–April 2015, Pages 167–171