Enhanced oxalic acid production from corncob by a methanol-resistant strain of Aspergillus niger using semi solid-sate fermentation

• The methanol-resistant Aspergillus niger KACC 44333 was generated.
• Oxalic acid production by mutant increased by 9.14 folds higher than the parent.
• Alkaline-pretreated corncob is a great potential carbon source.
• The highest production of oxalic acid was 123 g/kg dry weight of corncob.

Enhanced oxalic acid production was investigated in semi-solid state fermentation of methanol-resistant Aspergillus niger using corncob. Methanol-resistant A. niger was selected from the sector of colony showing phenotypically intact growth despite 5% methanol in solid medium (PDA) and finally obtained through the adaptation process for 22 weeks. Oxalic acid production of methanol-resistant A. niger was dramatically enlarged in liquid culture (CZB) by 9.14 folds compared with that of parent cells. Semi-solid state fermentation of corncob has been developed for the application of low-cost and environment friendly biomass waste to organic acid production. Oxalic acid production was dependent of inoculum size and initial substrate concentration, which was optimized at 106 spores/g dry weight and 5% (w/v) corncob in 0.1 N NaOH solution, respectively, which led to the maximum productivity (123.0 g/kg dry weight of corncob). Results demonstrate that methanol-resistant A. niger has great potentials for oxalic acid production from biomass.

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