کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
34269 | 45016 | 2015 | 7 صفحه PDF | دانلود رایگان |
• Cryptococcus flavus GB-1 produces CfCLE that efficiently degrades biodegradable plastics.
• The CfCLE-encoding gene, CfCLE1 was obtained and sequenced.
• A CfCLE productivity enhanced mutant C. flavus GB-1-DMC1 was obtained.
• Xylose-fed batch cultivation using a jar fermentor was useful to scale-up CfCLE production.
• CfCLE degraded various BPs cast films.
The yeast strain, Cryptococcus flavus GB-1, isolated from rice husks as a candidate producer of a biodegradable plastic (BP)-degrading enzyme. The genomic DNA sequence encoding the BP-degrading enzyme of GB-1 was determined. The enzyme was named CfCLE because the deduced amino acid sequence (239 amino acids) was found to be 93% identical to that of CLE of Cryptococcus sp. S-2 (accession No. BAK82405.1). The strain GB-1 forms a clear zone (halo) around its colonies on emulsified polybutylene succinate-co-adipate (PBSA) agar plates containing soybean oil but not on plates containing glucose. A UV-induced mutant from this strain, designated as GB-1-DMC1, formed halos on emulsified PBSA plates containing glucose and showed 1.53 U/ml of PBSA-degrading activity in liquid culture with glucose, which was more than 2.5-fold higher than that of the parent strain GB-1 (0.58 U/ml). Moreover, using xylose as a carbon source enhanced the PBSA-degrading activity of both strains. Xylose fed-batch cultivation of GB-1 and GB-1-DMC1 using a 5-l jar fermentor for 72 h produced 12.1 and 21.2 U/ml of CfCLE, respectively. Purified CfCLE has a molecular mass of 22 kDa, and its optimal pH and temperature for enzyme activity is 7.8 and 45 °C, respectively. CfCLE degraded various BP cast films.
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Journal: Process Biochemistry - Volume 50, Issue 11, November 2015, Pages 1718–1724