کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3446966 1595490 2012 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Alteration of Telomere Length and Subtelomeric Methylation in Human Endothelial Cell Under Different Levels of Hypoxia
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی پزشکی و دندانپزشکی (عمومی)
پیش نمایش صفحه اول مقاله
Alteration of Telomere Length and Subtelomeric Methylation in Human Endothelial Cell Under Different Levels of Hypoxia
چکیده انگلیسی

Background and AimsHypoxia-associated changes of telomeric structure in cell cultures have been analyzed mainly in cancer cells, stem cells, or cells transduced with vectors containing the telomerase gene, but not in somatic cells. The stability of telomere structure has been reported to be associated with subtelomeric methylation status. However, there are no reports of epigenetic alterations of telomeric regions of human somatic cells under hypoxia. This study aims at detecting and analyzing the subtelomeric methylation status in human somatic cells cultured under hypoxia.MethodsMean telomere length and telomerase activity of human umbilical vein endothelial cells (HUVECs) cultured in hypoxic conditions were measured. Subtelomeric methylation status of these cells was assessed by genomic Southern blot with telomere DNA probe using methylation-sensitive and -insensitive isoschizomers, MspI and HpaII.ResultsThe telomerase activity in HUVECs correlated inversely with the oxygen concentration. Mild hypoxia (10 or 15% oxygen) increased the telomere lengths, whereas the telomere lengths did not appear to change when <1% O2. The subtelomere of the shortest telomere range was methylated the most at 1% O2.ConclusionsSubtelomeric hypermethylation of short telomeres at 1% O2 compared to milder hypoxia implied that the subtelomeric hypermethylation may yield telomere stability and favor the cell survival of short telomere-bearing cells.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Archives of Medical Research - Volume 43, Issue 1, January 2012, Pages 15–20
نویسندگان
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