کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3447674 1595535 2006 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Overdiagnosis of Entamoeba histolytica and Entamoeba dispar in Nicaragua: A Microscopic, Triage Parasite Panel and PCR Study
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی پزشکی و دندانپزشکی (عمومی)
پیش نمایش صفحه اول مقاله
Overdiagnosis of Entamoeba histolytica and Entamoeba dispar in Nicaragua: A Microscopic, Triage Parasite Panel and PCR Study
چکیده انگلیسی

BackgroundBased on stool microscopy, an E. histolytica/E. dispar prevalence of 18.6% was found in León, Nicaragua about 10 years ago. Since then, new non-microscopic methods have been developed to discriminate between pathogenic E. histolytica and nonpathogenic E. dispar. The main objectives of the present study were to evaluate the true prevalence of E. histolytica among individuals with diarrhea and to assess the diagnostic procedures carried out at the health center level.MethodsA descriptive study was carried out on patients with diarrhea. Parasite detection was performed by conventional microscopy on native preparations or concentrated and stained specimens, Triage Parasite Panel and by PCR for both E. histolytica and E. dispar.ResultsIn 134 individuals with diarrhea, the prevalence of intestinal parasites was 69% as detected by direct stool examination. E. histolytica/E. dispar was found in eight (6%) of the samples, but the health centers reported 24%. In the Triage Parasite Panel only one case of E. histolytica/E. dispar was found. Analysis by PCR showed E. dispar in ten (7.5%) and E. histolytica in two cases (1.5%). The detection of intestinal coccidia and Dientamoeba fragilis required additional staining methods.ConclusionsPCR results showed that E. histolytica is a rare finding in patients with diarrhea. At the health centers, E. histolytica, E. histolytica/E. dispar were clearly overdiagnosed, with the consequence of overtreatment.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Archives of Medical Research - Volume 37, Issue 4, May 2006, Pages 529–534
نویسندگان
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