کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
34690 | 45039 | 2012 | 4 صفحه PDF | دانلود رایگان |
Burkholderia cepacia lipase was immobilized in silicates forming from n-butyl-substituted precursors within a silica monolith from methyl-substituted precursors. The resultant preparation gave about 12 times higher rates of transesterification of (R, S)-1-phenylethanol with vinyl acetate and an approximately two-fold increase in the enantioselectivity toward (R)-1-phenylethanol, as compared to a non-immobilized counterpart. The highest enzymatic activity and enantioselectivity (reaching 250) were found at a low water activity of 0.11. The continuous-flow kinetic resolution of (R, S)-1-phenylethanol was successfully conducted using lipase-immobilized silica monolith micro-bioreactors with various inside diameters ranging from 0.25 to 1.6 mm. The reactor performance during continuous operation was consistent with the prediction from the batch reactor. A steady state conversion of 40% and enantiomeric excess more than 98% were maintained over a time period of 15 days.
► Lipase-immobilized silica monolith is prepared using two-step sol–gel method.
► Lipase immobilized in butyl-silicate displays high activity and enantioselectivity.
► Silica monolith micro-bioreactor gives similar performance to batch reactor.
► Continuous-flow kinetic resolution is achieved stably with 98% enantiomeric excess.
Journal: Process Biochemistry - Volume 47, Issue 1, January 2012, Pages 147–150