کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
34695 | 45039 | 2012 | 5 صفحه PDF | دانلود رایگان |

Shewanella is a microbial genus that can oxidize lactate for the reduction of insoluble electron acceptors. This reduction is possible by either direct (cell–surface interaction, nanowires) or indirect (soluble redox mediators) mechanisms. However, the actual molecular identification of a nanowire has not been determined. Through mutational studies, Shewanella oneidensis MR-1 was analyzed for its ability to transfer electrons to an electrode after deletion of the structural pilin genes (ΔmshA-D) or the entire biosynthetic expression system (ΔmshH-Q) of one of its pilin complexes (Msh type IV pilus gene locus). The complete removal of the Msh complex (ΔmshH-Q) significantly decreased the current generated from a fuel cell compared to MR-1. However, the mutant with only extracellular Msh structural proteins removed (ΔmshA-D) was able to generate 80% of the current compared to MR-1. Thus, the intracellular and membrane bound Msh biogenesis complex is a pathway for extracellular electron transfer in S. oneidensis MR-1.
► Two S. oneidensis MR-1 mutants were analyzed for their ability to transfer electrons to an electrode.
► Complete removal of the Msh complex (ΔmshH-Q) significantly decreased the current generated from a fuel cell compared to MR-1.
► The mutant with only extracellular Msh structural proteins removed (ΔmshA-D) was able to generate 80% of the current compared to MR-1.
► Intracellular and membrane bound Msh biogenesis complex is a pathway for extracellular electron transfer in S. oneidensis MR-1.
Journal: Process Biochemistry - Volume 47, Issue 1, January 2012, Pages 170–174