کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
34701 45040 2014 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Bioprospecting of Amazon soil fungi with the potential for pigment production
ترجمه فارسی عنوان
بررسی زیست شناسی قارچ خاکی آمازون با پتانسیل تولید رنگدانه
کلمات کلیدی
قارچهای آمازون، رنگدانه ها، مشخصات شیمیایی، بهینه سازی
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
چکیده انگلیسی


• Fungi from Amazon soil are potential pigment producers.
• Five species stood out by the synthesis of coloured compounds.
• Sclerotiorin was isolated from Penicillium sclerotiorum 2AV2.
• Biocolourants demonstrate the importance of further bioprospecting studies in Amazon.

The aim of this study was to isolate fungi able to produce pigments. Fifty strains were isolated from the Amazon soil by the conventional technique of serial dilution. Submerged fermentation was performed in Czapeck broth in order to select strains able to synthesise pigments. Five strains were able to produce pigments and were identified by sequencing the rDNA (ITS regions). These fungi were identified as Penicillium sclerotiorum 2AV2, Penicillium sclerotiorum 2AV6, Aspergillus calidoustus 4BV13, Penicillium citrinum 2AV18 and Penicillium purpurogenum 2BV41. P. sclerotiorum 2AV2 produced intensely coloured pigments and were therefore selected for chemical characterisation. NMR identified the pigment as sclerotiorin. In this work, the influence of nutrients on sclerotiorin yield was also studied and it was verified that rhamnose and peptone increased production when used separately. These results indicate that Amazonian fungi bioprospecting is a viable means to search for new sources of natural dyes.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Process Biochemistry - Volume 49, Issue 4, April 2014, Pages 569–575
نویسندگان
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