کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
34819 | 45051 | 2014 | 8 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Direct bioconversion of d-xylose to 1,2,4-butanetriol in an engineered Escherichia coli Direct bioconversion of d-xylose to 1,2,4-butanetriol in an engineered Escherichia coli](/preview/png/34819.png)
• Single microbial conversion of d-xylose to 1,2,4-butanetriol is first reported.
• Pathway thermodynamics, product toxicity and redox in E. coli were investigated.
• Expression of two genes from two different bacteria completes the four-step pathway.
• Highest titer achieved upon deletion of native d-xylose and d-xylonate metabolism.
The compound 1,2,4-butanetriol (BT) is a valuable chemical used in the production of plasticizers, polymers, cationic lipids and other medical applications, and is conventionally produced via hydrogenation of malate. In this report, BT is biosynthesized by an engineered Escherichia coli from d-xylose. The pathway: d-xylose → d-xylonate → 2-keto-3-deoxy-d-xylonate → 3,4-dihydroxybutanal → BT, was constructed in E. coli by recruiting a xylose dehydrogenase and a keto acid decarboxylase from Caulobacter crescentus and Pseudomonas putida, respectively. Authentic BT was detected from cultures of the engineered strain. Further improvement on the strain was performed by blocking the native d-xylose and d-xylonate metabolic pathways which involves disruption of xylAB, yjhH and yagE genes in the host chromosome. The final construct produced 0.88 g L−1 BT from 10 g L−1d-xylose with a molar yield of 12.82%. By far, this is the first report on the direct production of BT from d-xylose by a single microbial host. This may serve as a starting point for further metabolic engineering works to increase the titer of BT toward industrial scale viability.
Journal: Process Biochemistry - Volume 49, Issue 1, January 2014, Pages 25–32