کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3485103 1596911 2016 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Bioinformatics microarray analysis and identification of gene expression profiles associated with cirrhotic liver
ترجمه فارسی عنوان
تجزیه و تحلیل میکروارگانی بیوانفورماتیک و شناسایی پروفایل های بیان ژن در ارتباط با کبد سیروز کبدی
کلمات کلیدی
بیوانفورماتیک، پروفیل های ژنی، کبد، سیروز کبدی، تجزیه و تحلیل میکروارا
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی پزشکی و دندانپزشکی (عمومی)
چکیده انگلیسی

Cirrhosis is the endpoint of liver fibrosis that is accompanied by limited regeneration capacity and complications and is the ultimate cause of death in many patients. Despite this, few studies have thoroughly looked at the gene expression profiles in the cirrhotic liver. Hence, this study aims to identify the genes that were differentially expressed in the cirrhotic liver and to explore the putative related signaling pathway and interaction networks. The gene expression profiles of cirrhotic livers and noncirrhotic livers were examined and compared using microarray gene analysis. Proteins encoded by the differentially expressed genes were analyzed for functional clustering and signaling pathway involvement using MetaCore bioinformatics analyses. The Gene Ontology analysis as well as the Kyoto encyclopedia of Genes and Genomes pathway analysis were also performed. A total of 213 significant genes were differentially expressed at more than a two-fold change in cirrhotic livers as compared to noncirrhotic livers. Of these, 105 upregulated genes and 63 downregulated genes were validated through MetaCore bioinformatics analyses. The signaling pathways and major functions of proteins encoded by these differentially expressed genes were further analyzed; results showed that the cirrhotic liver has a unique gene expression pattern related to inflammatory reaction, immune response, and cell growth, and is potentially cancer related. Our findings suggest that the microarray analysis may provide clues to the molecular mechanisms of liver cirrhosis for future experimental studies. However, further exploration of areas regarding therapeutic strategy might be possible to support metabolic activity, decrease inflammation, or enhance regeneration for liver cirrhosis.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The Kaohsiung Journal of Medical Sciences - Volume 32, Issue 4, April 2016, Pages 165–176
نویسندگان
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