کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
34863 | 45052 | 2013 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Preparative method for isosilybin isolation based on enzymatic kinetic resolution of silymarin mixture Preparative method for isosilybin isolation based on enzymatic kinetic resolution of silymarin mixture](/preview/png/34863.png)
The flavonolignan isosilybin (1) is one of the silybin congeners contained in the silymarin complex, which is isolated from the seeds of the milk thistle (Silybum marianum). A number of recent studies have demonstrated that isosilybin is probably the most potent anticancer agent found in silymarin. It occurs as a mixture of two diastereoisomers, A and B. Lipase Novozym 435 was found to allow the preparative production of both optically pure isosilybin A and B in a diastereoisomeric purity of over 95%. A preparatory method based on the enzymatic resolution and other purification methods has been developed, enabling multigram amounts of both optically pure isosilybins A (1a) and B (1b) to be obtained.
► Isosilybin is probably the most potent anticancer compound in silymarin.
► Preparatory method for isolation of optically pure isosilybin A and B was developed.
► A core of the method is diastereomeric discrimination with lipase Novozym 435.
► Other pure silymarin components, such as taxifolin and silydianin can be obtained.
Journal: Process Biochemistry - Volume 48, Issue 1, January 2013, Pages 184–189