کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
35058 45071 2009 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Purification of lipase derived from Burkholderia pseudomallei with alcohol/salt-based aqueous two-phase systems
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Purification of lipase derived from Burkholderia pseudomallei with alcohol/salt-based aqueous two-phase systems
چکیده انگلیسی

Alcohol/salt-based aqueous two-phase systems (ATPSs) were used to recover lipase derived from Burkholderia pseudomallei (B. pseudomallei). Nine biphasic systems, comprised of an alcohol-based top phase (ethanol, 2-propanol and 1-propanol) and a salt-based bottom phase (ammonium sulfate, potassium phosphate and sodium citrate), were evaluated for their effectiveness in lipase recovery. The stability of lipase in each of the solutions was tested, and phase diagrams were constructed for each system. The optimum partition efficiency for the purification of lipase was obtained in an ATPS of 16% (w/w) 2-propanol and 16% (w/w) phosphate in the presence of 4.5% (w/v) NaCl. The purified lipase had a purification factor of 13.5 and a yield of 99%.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Process Biochemistry - Volume 44, Issue 10, October 2009, Pages 1083–1087
نویسندگان
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