کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
35099 45074 2009 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Lipase immobilization on O-propargyl and O-pentynyl dextrans and its application for the synthesis of click beetle pheromones
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Lipase immobilization on O-propargyl and O-pentynyl dextrans and its application for the synthesis of click beetle pheromones
چکیده انگلیسی

Lipase of Rhizopus arrhizus was immobilized on O-propargyl dextran (PgD) and O-pentynyl dextran (PyD). Compared with Lewatit VP OC 1600 cation ion exchange resin, wood shaves, fuller earth, silica and alumina, PgD with degree of substitution (DS) of 0.68 and a surface of 10 m2/g was found to be the most effective immobilization support and an excellent biocatalyst for esterification reactions in organic solvents as the synthesis of click beetle pheromone geranyl octanoate. PyD (DS 0.44) with a surface of 3.3 m2/g was of similar high efficiency. For the enzymatic esterification the optimum concentration of geraniol and octanoic acid was 0.4 mol L−1 each. The biocatalyst worked the best in hexane at a moisture level of 0.02%. The enzyme could be repeatedly used and conversion dropped from 80% to 70% after four cycles, while reaction rate even increased when repeatedly employed.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Process Biochemistry - Volume 44, Issue 11, November 2009, Pages 1276–1283
نویسندگان
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