کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
35153 45078 2012 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Combination use of ultrasound irradiation and ionic liquid in enzymatic isomerization of glucose to fructose
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Combination use of ultrasound irradiation and ionic liquid in enzymatic isomerization of glucose to fructose
چکیده انگلیسی

The synthetic effect of the combination use of ultrasound irradiation (UI) and ionic liquid (IL) on improving enzyme activity was studied when they were employed in isomerization of glucose to fructose by immobilized glucose isomerase (IGI, produced from streptomyces murinus and immobilized on silica). Both of UI and IL [EMIM][Cl], which was screened as the best medium for this reaction, were found to increase the enzyme activity in isomerization reaction. And a further increase of enzyme activity was observed by combination use of UI and IL. A systematic screening and optimization of the reaction parameters in ILs under UI on the IGI activity were performed. Under the optimum reaction conditions, 45.3% yield of fructose was achieved in 10 h under UI in [EMIM][Cl], compared to only 41.5% yield under stirring in [EMIM][Cl], 44.2% under UI without [EMIM][Cl] and 38.9% under stirring without [EMIM][Cl] in 12 h, respectively. High thermal stability and reusability of IGI was also observed under UI in [EMIM][Cl]. These results indicated that the combination use of UI and IL might be a fast and efficient method for enzymatic isomerization of glucose to fructose.


► Combination use of ultrasound irradiation and ionic liquid in enzymatic isomerization reaction.
► A synergistic effect of ultrasound irradiation and ionic liquid on improving enzyme activity.
► Increased thermal stability and reusability of isomerase.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Process Biochemistry - Volume 47, Issue 6, June 2012, Pages 976–982
نویسندگان
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