Hydrolysis optimization of mannan, curdlan and cell walls from Endomyces fibuliger grown in mussel processing wastewaters

The aim of this report was to optimize the hydrolysis of the cell walls (CWs) from the yeast Endomyces fibuliger grown in mussel processing wastewaters (MPW) to establish a more accurate protocol for analyzing the composition of the monosaccharides in these CWs. Therefore, a kinetic study of CW hydrolysis and polysaccharide standards (mannan and curdlan) was performed to determine the effect of different temperatures and trifluoroacetic acid (TFA) concentrations on this process. In all cases, the experimental data were fit satisfactorily to Saeman's equation with an Arrhenius relation between rate constants and the temperature effect. Optimal conditions for curdlan and mannan hydrolysis were achieved with 70% TFA at 100 °C for 2.3 h and 50% TFA at 100 °C for 2.6 h, respectively. The best operating options for CW hydrolysis were 100 °C/70% TFA for 4.58 h, 100 °C/50% TFA for 4.08 h and 100 °C/70% TFA for 3.27 h for the maximum production of glucose, mannose and reducing sugars, respectively.