کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
35272 45084 2009 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Characterization of thermo- and detergent stable serine protease from isolated Bacillus circulans and evaluation of eco-friendly applications
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Characterization of thermo- and detergent stable serine protease from isolated Bacillus circulans and evaluation of eco-friendly applications
چکیده انگلیسی

Alkaline protease from Bacillus circulans has been purified and characterized in detail for its robustness and its eco-friendly application potential at leather processing and detergent industries. The molecular weight of the purified enzyme was estimated to be 39.5 kDa on SDS-PAGE. It exhibited optimum activity at broad temperature range and maximum at 70 °C under alkaline pH environment, in the presence of surfactants and oxidizing agents. It has revealed stain removal property and dehairing activity for animal hide without chemical assistance and without hydrolyzing fibrous proteins. This enzyme showed application potential in leather processing industry for production of better quality product in eco-friendly process. In addition, the stability (pH, temperature and surfactants) and hydrolysis of blood stain data also revealed its application in detergent industries.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Process Biochemistry - Volume 44, Issue 3, March 2009, Pages 262–268
نویسندگان
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