کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
35291 | 45084 | 2009 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: A putative secreted solute binding protein, SCO6569 is a possible AfsR2-dependent down-regulator of actinorhodin biosynthesis in Streptomyces coelicolor A putative secreted solute binding protein, SCO6569 is a possible AfsR2-dependent down-regulator of actinorhodin biosynthesis in Streptomyces coelicolor](/preview/png/35291.png)
AfsR2 is a global regulatory protein involved in stimulating the biosynthesis of antibiotics in various Streptomyces species. Using 2-D gel electrophoresis and MALDI-TOF analyses, several putative AfsR2-target proteins were previously identified. In order to verify the biological significance of the proteomics-guided targets, the sixteen putative AfsR2 target genes were individually cloned under the strong-and-constitutive promoter (PermE*) of the Streptomyces expression vector, pSE34, followed by the transformation into both Streptomyces lividans and Streptomyces coelicolor. Among the putative AfsR2-target genes tested, the expression of a putative secreted solute binding protein, SCO6569 significantly repressed biosynthesis of actinorhodin in S. coelicolor, and the transcripts encoded by the pathway-specific activator, actII-ORF4 for actinorhodin were also reduced in SCO6569-overexpressing S. coelicolor. Moreover, SCO6569 gene-disruption in S. coelicolor further enhanced actinorhodin production, suggesting that the SCO6569 could be a possible AfsR2-dependent down-regulator acting in actinorhodin biosynthesis in S. coelicolor.
Journal: Process Biochemistry - Volume 44, Issue 3, March 2009, Pages 373–377