کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
35304 45085 2009 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Use of the contour approach for visualizing the dynamic behavior of intermediates during O-nitrophenyl-β-d-galactoside hydrolysis by β-galactosidase
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Use of the contour approach for visualizing the dynamic behavior of intermediates during O-nitrophenyl-β-d-galactoside hydrolysis by β-galactosidase
چکیده انگلیسی

ONPG disappearance and ONP appearance were synchronously measured during ONPG hydrolysis by β-galactosidase using in situ on-line UV–vis spectroscopy. Intermediate formation was determined by the formula d[ONPG]/dt − d[ONP]/dt. The combined effects of temperature and time on νinst and νinc during the conversion of ONPG to ONP were expressed by the isogram method in which contour plots were used. Based on this approach, new insights were obtained into the irreversible-continuous conversion of ONPG to ONP during hydrolysis. The intermediate was a moving mass that flowed in three-dimensional space from the substrate to the product. The results of this study support the use of the isogram method for understanding the mechanisms of enzyme-catalyzed reactions via the dynamic resolution approach.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Process Biochemistry - Volume 44, Issue 12, December 2009, Pages 1374–1380
نویسندگان
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