کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
35522 45093 2010 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Insoluble complex formation between alpha-amylase from Aspergillus oryzae and polyacrylic acid of different molecular weight
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Insoluble complex formation between alpha-amylase from Aspergillus oryzae and polyacrylic acid of different molecular weight
چکیده انگلیسی

The insoluble complex formation between alpha-amylase and the strong anionic polyelectrolyte polyacrylic acid was studied by using turbidimetric and enzymatic activity. The highest molecular weight polyacrylic acid (100,000 Da and 240,000 Da) proved to be suitable precipitating agents. They were insoluble at pH lower than 4–5, with a stoichiometric ratio polymer mol per protein mol of 1:52 and 1:154, respectively. Electrostatic interactions are not the only factor in the formation of insoluble complexes. High percentage of alpha-amylase enzymatic activity maintains throughout time, even in the presence of polyelectrolyte.The application of precipitation conditions found when applying a bovine homogenate showed that it is not suitable for purification even if it proved to be useful methodology for the concentration of the enzyme and can be used as a first step of purification.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Process Biochemistry - Volume 45, Issue 10, October 2010, Pages 1753–1756
نویسندگان
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