کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
35698 45102 2007 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Evaluation of recombinant phenylalanine dehydrogenase behavior in aqueous two-phase partitioning
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Evaluation of recombinant phenylalanine dehydrogenase behavior in aqueous two-phase partitioning
چکیده انگلیسی

Recombinant Bacillus sphaericus phenylalanine dehydrogenase (PheDH) partitioning was studied in polyethylene glycol (PEG) and ammonium sulfate aqueous two-phase systems (ATPS). The objectives of this work were to investigate influences; varying the molecular mass and concentration of PEG, pH, phase volume ratio (VR), tie-line length (TLL) and concentration of (NH4)2SO4 on the partition behavior of PheDH. It was revealed that the partitioning was not affected by VR, while PEG molecular mass and concentration and (NH4)2SO4 concentration had significant effects on enzyme partitioning. Longer TLL and higher pH resulted in better partitioning into the top phase. Under the most favorable partition conditions with 8.5% (w/w) PEG-6000, 17.5% (w/w) (NH4)2SO4 and VR = 0.25 at pH 8.0, partition coefficient (KE), recovery (R%), yield (Y%) and TLL were achieved 58.7%, 135%, 94.42% and 39.89% (w/w), respectively. Overall, the promising results obtained in this research indicated that the ATPS partitioning can be provided an efficient and powerful tool for recovery and purification of recombinant PheDH.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Process Biochemistry - Volume 42, Issue 9, September 2007, Pages 1296–1301
نویسندگان
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