کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
35924 45114 2008 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Jasmonic acid mediates gene transcription of ginsenoside biosynthesis in cell cultures of Panax notoginseng treated with chemically synthesized 2-hydroxyethyl jasmonate
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Jasmonic acid mediates gene transcription of ginsenoside biosynthesis in cell cultures of Panax notoginseng treated with chemically synthesized 2-hydroxyethyl jasmonate
چکیده انگلیسی

The highly efficient induction of ginsenoside biosynthesis of Panax notoginseng cells by newly synthesized elicitors such as 2-hydroxyethyl jasmonate (HEJ, at 200 μM) was found previously. In this work, three cDNA fragments of genes encoding squalene synthase (SQS), squalene epoxidase (SE) and cycloartenol synthase (CAS) related to triterpene (including ginsenoside) biosynthesis were cloned from P. notoginseng cells, and those genes’ transcription was investigated in the cell cultures elicited by HEJ. Upon HEJ or MJ treatment, the induction of endogenous jasmonic acid (JA) biosynthesis, up-regulation of SQS and SE genes and down-regulation of CAS gene were all observed. A JA biosynthetic inhibitor, diethydithiocarbamate, could effectively inhibit the JA biosynthesis and depress the HEJ-induced up-regulation of SQS and SE genes’ expression and down-regulation of CAS gene expression, and the ginsenoside biosynthesis was simultaneously inhibited. The results suggested that JA as a signal transducer played an important role in the ginsenoside biosynthesis by P. notoginseng cells. The information is useful to further manipulation and understanding of ginseng saponin biosynthesis in plant cell cultures.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Process Biochemistry - Volume 43, Issue 1, January 2008, Pages 113–118
نویسندگان
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