کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
35943 | 45115 | 2006 | 4 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Microbacterium trichotecenolyticum enzyme mediated transformation of arteannuin B to artemisinin Microbacterium trichotecenolyticum enzyme mediated transformation of arteannuin B to artemisinin](/preview/png/35943.png)
Artemisinin, a sesquiterpene lactone containing an endoperoxide bridge, isolated from Artemisia annua L. is effective against both drug resistant and cerebral malaria causing strains of Plasmodium falciparum. The relative low yields of artemisinin in plants are a serious limitation to the commercialization of the drug. An alternative approach by microbial bioconversion of arteannuin B to artemisinin was carried out by Microbacterium trichotecenolyticum isolated from soil. Crude enzyme extract from cell free extracts were capable of microbial bioconversion of arteannuin B, the immediate precursor of artemisinin, to artemisinin. Attempts have been made to partially purify the proteins involved in bioconversion by ion exchange chromatography. Detection of artemisinin was done by thin layer chromatography, and quantified by HPLC.
Journal: Process Biochemistry - Volume 41, Issue 12, December 2006, Pages 2464–2467