کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
36141 | 45122 | 2007 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Immobilization of urease on vapour phase stain etched porous silicon
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Porous silicon layers fabricated by the reaction-induced vapor phase stain etch method were coated with 5% polyethylenimine. Urease from Canavalia brasiliensis beans was immobilized on this support through covalent linking with 2.5% glutaraldehyde. The pH and temperature profile of the immobilized and free urease exhibited higher activity at pH 6.5 and 37 °C. After being stored for 30 days at 4 °C, the immobilized enzyme had 75% of the initial activity. The maximum apparent Michaelis constant for free urease (Km) was 94.33 mM whereas for immobilized urease was 53.04 mM. The maximum reaction velocity (Vmax) for free urease was 3.51 mmol/min and for immobilized urease was 1.57 mmol/min.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Process Biochemistry - Volume 42, Issue 3, March 2007, Pages 429–433
Journal: Process Biochemistry - Volume 42, Issue 3, March 2007, Pages 429–433
نویسندگان
Maria de Mascena Diniz Maia, Elder Alpes de Vasconcelos, Paula Ferdinanda Conceição de Mascena Diniz Maia, Jackeline da Costa Maciel, Karla Regina Rizzardi Cajueiro, Maria da Paz Carvalho da Silva, Eronides Felisberto da Silva Jr,