کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
36250 | 45125 | 2005 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Enzymic synthesis of salidroside: Purification and characterization of salidrosidase from Aspergillas niger
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
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چکیده انگلیسی
An enzyme synthesising salidroside with p-tyrosol and β-d-glucose was isolated from Aspergillas niger, purified and characterized in this paper. After (NH4)2SO4 precipitation and ion exchange chromatography, the enzyme was purified to one band in SDS-polyacrylamide gel electrophoresis, and its molecular weight was about 84.6 kDa. The optimum temperature of the enzyme was 45 °C, and the optimum pH was 5.0. The enzyme was stable at less than 55 °C and pH 4.5–6.0. The enzyme catalyzed p-tyrosol and β-d-glucose to form salidroside in the concentrations of 1.5% p-tyrosol and 6% β-d-glucose. The yield of salidroside was about 10%.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Process Biochemistry - Volume 40, Issue 9, September 2005, Pages 3143–3147
Journal: Process Biochemistry - Volume 40, Issue 9, September 2005, Pages 3143–3147
نویسندگان
Chunzhi Zhang, Hongshan Yu, Mingchun Lu, Jing Li, Fengxie Jin,