کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
36274 45127 2006 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Partitioning of the glucoamylase activity at the cell surfaces in cultures of Saccharomyces
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Partitioning of the glucoamylase activity at the cell surfaces in cultures of Saccharomyces
چکیده انگلیسی

Glucoamylases have been used with α-amylases for the industrial conversion of starch into glucose. However, little is known about the properties of this glycosylated protein retained in the cell wall of Saccharomyces as well as its role in the saccharification and fermentation of amylaceous substrates, notably in high cell density processes. In most of the strains assayed, decreases in biomass formation were followed by increases in glucoamylase secretion (expressed as U/mgbiomass in 1 ml of culture) when glucose was exchanged for starch as carbon source or the growth temperature was raised from 30 to 35 °C. Despite the losses in viability, significant increases in the activity of the wall fraction occurred when cultures of thermotolerant yeasts propagated at 30 °C or washed cells resuspended in buffer solution were heated to 60 °C for 60–80 min prior to amylolytic assays. Thus, intact cells of thermotolerant yeasts can be used as colloidal biocatalysts in starch degradation processes.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Process Biochemistry - Volume 41, Issue 1, January 2006, Pages 20–27
نویسندگان
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