کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
36601 | 45138 | 2006 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Covalent immobilization of triacylglycerol lipase onto functionalized nanoscale SiO2 spheres
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Covalent immobilization of triacylglycerol lipase onto functionalized nanoscale SiO2 spheres Covalent immobilization of triacylglycerol lipase onto functionalized nanoscale SiO2 spheres](/preview/png/36601.png)
چکیده انگلیسی
Three novel nano-silica dioxide (NSD) carriers with reactive aldehyde groups, i.e. NSD-1, NSD-2 and NSD-3, were firstly prepared through functionalization of the NSD material, and porcine pancreas lipase (PPL) was immobilized on the resulting carriers by using covalent coupling method. The activity recovery of immobilized PPL based on the carrier NSD-1, NSD-2 and NSD-3 reaches to 73%, 79% and 118%, respectively, and the values of apparent KM of them were all higher than that of the free PPL. The optimal pH and temperature region of the immobilized PPLs for the hydrolysis of olive oil were at 8.0 and 50–60 °C. Meanwhile, the immobilized PPLs have a good stability, adaptability and reusability.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Process Biochemistry - Volume 41, Issue 4, April 2006, Pages 770–777
Journal: Process Biochemistry - Volume 41, Issue 4, April 2006, Pages 770–777
نویسندگان
Yong-Xiao Bai, Yan-Feng Li, Yong Yang, Liu-Xiang Yi,