کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
36684 45142 2005 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Chemical and immunological differentiation of exoantigens from four Bipolaris sorokiniana strains
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Chemical and immunological differentiation of exoantigens from four Bipolaris sorokiniana strains
چکیده انگلیسی

Four strains (3, 5, 8, and 18) of Bipolaris sorokiniana were assessed as to their antigenic and partial chemical composition through antisera produced in rabbits. The antisera were immunochemically analyzed by double diffusion and immunoelectrophoresis. The partial chemical characterization of exoantigens was obtained by measuring the amount of proteins, carbohydrates and lipids in the strains. Immunoelectrophoresis showed that strain 18 antigens stimulated the production of nine precipitin bands. No significant differences were found between the antisera produced by the animals immunized with the same antigen. Partial chemical analysis showed protein content between 173.4 and 332.2 μg mL−1; carbohydrate levels between 0 and 64.3 μg mL−1; and lipid content from 0 to 166 μg mL−1. Immunodiffusion and immunoelectrophoresis associated with chemical characterization are relevant when phenotypic traits of different strains of the same fungus species are studied.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Process Biochemistry - Volume 40, Issue 6, May 2005, Pages 2051–2057
نویسندگان
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