کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3883177 1249396 2012 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The C5a receptor has a key role in immune complex glomerulonephritis in complement factor H–deficient mice
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی بیماری‌های کلیوی
پیش نمایش صفحه اول مقاله
The C5a receptor has a key role in immune complex glomerulonephritis in complement factor H–deficient mice
چکیده انگلیسی

Chronic serum sickness leads to the formation of glomerular immune complexes; however, C57BL/6 mice do not develop glomerulonephritis unless complement factor H (CFH) is absent from the plasma. Here we studied the role for C5a receptor (R) in this setting. The exaggerated humoral immune response in CFH−/− mice was normalized in CFH−/−C5aR−/− double knockout mice, highlighting the C5aR dependence. The CFH knockout mice developed proliferative glomerulonephritis with endocapillary F4/80+ macrophage infiltration, a process reduced in the double knockout mice. There was no interstitial inflammation by histologic criteria or flow cytometry for F4/80+Ly6ChiCCR2hi inflammatory macrophages. There were, however, more interstitial CD3+CD4+ T lymphocytes in CFH knockout mice with chronic serum sickness, while double knockout mice had greater than 5-fold more Ly6CloCCR2lo anti-inflammatory macrophages compared to the CFH knockout mice. Mice lacking C5aR were significantly protected from functional renal disease as assessed by blood urea nitrogen levels. Thus, IgG- and iC3b-containing immune complexes are not inflammatory in C57BL/6 mice. Yet when these mice lack CFH, sufficient C3b persists in glomeruli to generate C5a and activate C5aR.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Kidney International - Volume 82, Issue 9, 1 November 2012, Pages 961–968
نویسندگان
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