کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3886114 1249535 2006 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Reactive oxygen species deglycosilate glomerular α-dystroglycan
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی بیماری‌های کلیوی
پیش نمایش صفحه اول مقاله
Reactive oxygen species deglycosilate glomerular α-dystroglycan
چکیده انگلیسی

In the kidney, dystroglycan (DG) has been shown to cover the basolateral and apical membranes of the podocyte. α-DG is heavily glycosilated, which is important for its binding to laminin and agrin in the glomerular basement membrane. Furthermore, α-DG is negatively charged, which maintains the filtration slit open. Reactive oxygen species (ROS) are known to degrade and depolymerize carbohydrates, and to play a role in several glomerular diseases. Therefore, we evaluated the effect of ROS on the glycosilation of glomerular α-DG. By using specific antibodies directed against the core protein or glyco-epitopes of α-DG, this was studied in a solid-phase assay, in situ on kidney sections, and in vivo in adriamycin nephropathy. A ligand overlay assay was used to study binding of α-DG to its ligands. Exposure to ROS leads to a loss of carbohydrate epitopes on α-DG both in vitro and on kidney sections. In the in vitro assays, a decreased binding of deglycosilated α-DG to laminin and agrin was found. In adriamycin nephropathy, where radicals play a role, we observed a loss of α-DG carbohydrate epitopes. We conclude that deglycosilation of glomerular α-DG by ROS leads to disruption of the agrin–DG complex, which in vivo may lead to the detachment of podocytes. Furthermore, loss of negative charge in the filtration slit may lead to foot process effacement of podocytes.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Kidney International - Volume 69, Issue 9, 1 May 2006, Pages 1526–1534
نویسندگان
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