کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3909020 1251201 2012 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Inter- and intra-tumoral heterogeneity in DNA damage evaluated by comet assay in early breast cancer patients
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی زنان، زایمان و بهداشت زنان
پیش نمایش صفحه اول مقاله
Inter- and intra-tumoral heterogeneity in DNA damage evaluated by comet assay in early breast cancer patients
چکیده انگلیسی

There are no clinical tools to functionally assess degree of DNA damage in breast cancer. The comet assay is an accepted research tool for assessing DNA damage, however, most cancer studies have assessed lymphocytes as surrogate cells. The aim of this pilot study was to use the comet assay in early breast cancer directly in tumor tissue to compare DNA damage between and within traditionally defined subgroups, and to explore intra-tumoral heterogeneity. Scrapings of tumor and healthy breast tissue were obtained at primary surgery from 104 women. Comet assay was applied to quantitatively assess DNA damage, revealing substantial inter- and intra-subgroup variation. Marked intra-tumoral heterogeneity was evident across all subgroups. The degree of DNA damage for an individual could not be predicted by breast cancer subgroup. Comet assay warrants further study as a potential clinical tool for identification of tumoral DNA damage and ultimately, individualised use of DNA damaging therapy.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The Breast - Volume 21, Issue 3, June 2012, Pages 336–342
نویسندگان
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