کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3916219 1251540 2006 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Evaluation of single intratesticular injection of calcium chloride for nonsurgical sterilization in adult albino rats
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی زنان، زایمان و بهداشت زنان
پیش نمایش صفحه اول مقاله
Evaluation of single intratesticular injection of calcium chloride for nonsurgical sterilization in adult albino rats
چکیده انگلیسی

ObjectiveTo investigate a method of chemical sterilization and its efficacy in adult albino rats.MethodEvaluation was conducted 3 weeks after a single bilateral intratesticular injection of calcium chloride (CaCl2) at the dose of 5, 10, 15 or 20 mg per testis per 100 g body weight.ResultsThe significant graded diminution in relative sex organ weights, testicular androgenic enzymes like Δ5,3β-hydroxysteroid dehydrogenase (Δ5,3β-HSD) and 17β-hydroxysteroid dehydrogenase (17β-HSD), glutathione peroxidase (GPx), glutathione S-transferase (GST) and superoxide dismutase (SOD) activities, testicular content of reduced glutathione (GSH), plasma and intratesticular concentrations of testosterone, epididymal sperm count as well as significant elevation in plasma concentrations of luteinizing hormone (LH), follicle-stimulating hormone (FSH) and testicular content of malondialdehyde (MDA) were noted in all the treated groups with respect to vehicle control. There was no chronic general stress in experimental animals as indicated by insignificant changes in plasma concentrations of corticosterone, prolactin, total protein, blood urea nitrogen and fasting blood sugar level. Dose-dependent responses on testicular histopathology were recorded by noting multinucleated giant cells in seminiferous tubules, derangement of tubular architecture along with infiltration of leucocytes and appearance of fibrous tissue throughout the testicular sections. The fertility efficacy of the 10, 15 or 20 mg CaCl2-treated males was nil, proven after mating with fertile, virgin healthy females, as there were no implantation sites in each uterine horns noted by laparotomy.ConclusionIntratesticular CaCl2 injection at a specific dose might serve as a way of sterilization and may be considered as an alternative to surgical castration in male animals.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Contraception - Volume 73, Issue 3, March 2006, Pages 289–300
نویسندگان
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