کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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3936598 | 1253453 | 2009 | 10 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Effects of cigarette smoke on fertilization and embryo development in vivo Effects of cigarette smoke on fertilization and embryo development in vivo](/preview/png/3936598.png)
ObjectiveTo determine the effects of smoking on eggs and subsequent embryo development by maternal exposure to cigarette smoke.DesignMice were exposed to cigarette smoke or cigarette smoke condensate (CSC) for 4 weeks and then examined for development and telomere function of embryos in vitro after fertilization. In addition, the effects of continuous smoke on embryo development and telomere length were determined by treating mice for 4 weeks, followed by continous exposure to cigarette smoke or CSC after fertilization.SettingLaboratory study.Animal(s)CD1 mice.Intervention(s)Mice were exposured to cigarette smoke or CSC.Main Outcome Measure(s)The percentage (rate) of blastocyst development, quality of embryos assessed by total cell number, apoptosis, Oct4 expression (a molecular marker of embryonic stem cells), telomere length and loss, and chromosomal instability were compared between smoke- and CSC- treated mice and sham-treated mice.Result(s)Mice exposed to cigarette smoke or CSC for 4 weeks exhibited increased egg fragmentation or delayed fertilization, thus reducing development to blastocysts in vitro. Fragmented eggs showed increased reactive oxygen species. Mice exposed to smoke or CSC showed increased apoptosis and altered expression of Oct4 in developed embryos. The effects of smoke or CSC on embryo development showed a dose-dependent relationship to exposure time. Exposure to smoke or CSC beginning 4 weeks before fertilzation altered expression of Oct4 and increased apoptosis in blastocysts. Notably, the rate of abnormal embryos significantly increased in the smoke and CSC groups. Smoke and CSC shortened telomeres in embryos, but their telomere shortening was not enough to induce major chromosome abnormalities in mice, which have unusually long telomeres.Conclusion(s)Together, the whole animal exposure model shows that cigarette smoke induces oxidative stress, telomere shortening, and apoptosis, and compromises embryo development in vivo.
Journal: Fertility and Sterility - Volume 92, Issue 4, October 2009, Pages 1456–1465