کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3939073 1253548 2008 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Computer-assisted motility analysis of spermatozoa obtained after spermatogonial stem cell transplantation in the mouse
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی زنان، زایمان و بهداشت زنان
پیش نمایش صفحه اول مقاله
Computer-assisted motility analysis of spermatozoa obtained after spermatogonial stem cell transplantation in the mouse
چکیده انگلیسی

ObjectiveTo study the motility characteristics of epididymal spermatozoa after spermatogonial stem cell transplantation.DesignTesticular cells from fertile donor mice were transplanted to the testis of genetically sterile recipient mice. Three to nine months later, the epididymal spermatozoa were isolated and used for a computer-assisted sperm motility analysis. Spermatozoa from fertile adult mice were used as control.SettingMurine transplantation model in an academic research environment.Animal(s)Donors, 6-day-old male C57Bl × WBRej F1 mice; acceptors, 4- to 6-week-old W/Wv mice of the same genetic background.Intervention(s)Two to 10 μL from a 30-million/mL testicular cell suspension was injected through the efferent duct in the rete testis.Main Outcome Measure(s)Vitality, concentration, motility, individual sperm movement, and hyperactivity of the spermatozoa.Result(s)Vitality was comparable between the two groups; the concentration, motility, and hyperactivity of posttransplantation spermatozoa were significantly reduced. The movement pattern of the individual spermatozoon was normal at the time of isolation but decreased more rapidly during in vitro culture, compared with the case in controls. This difference already had reached a significant level 3 hours after culture, which is comparable with the duration of an IVF procedure.Conclusion(s)The reduced fertilization rate after IVF can thus be explained by a lower number of motile spermatozoa and the faster decrease of the individual sperm movement parameters.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Fertility and Sterility - Volume 90, Issue 4, Supplement, October 2008, Pages 1411–1416
نویسندگان
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