Selective accumulation of PpIX and photodynamic effect after aminolevulinic acid treatment of human adenomyosis xenografts in nude mice

ObjectiveTo evaluate the effect of photodynamic therapy with aminolevulinic acid (ALA) on human adenomyosis xenografts in a mouse model.DesignHuman adenomyosis tissues were implanted SC into nude mice. We measured 5-aminolevulinic acid pharmacokinetics in these mice by analyzing tissue sections 1 to 6 hours after intraperitoneal administration. Twenty-four hours after photodynamic therapy, we evaluated tissue morphologic features.SettingDepartment of obstetrics and gynecology at a university hospital in Japan.Patient(s)Immunodeficient mice. Tissue grafts were taken from women with adenomyosis attending a university hospital.Intervention(s)Photodynamic treatment.Main Outcome Measure(s)Peak fluorescence after intraperitoneal ALA administration and tissue histological changes 24 hours after photodynamic therapy.Result(s)Peak fluorescence was observed 3 hours after intraperitoneal administration. Histological studies revealed decreased numbers of epithelial and stromal cells in adenomyosis models after therapy.Conclusion(s)Photodynamic therapy with ALA caused extensive cell death in human adenomyosis tissues implanted into nude mice. Photodynamic treatment using ALA is a potential treatment for patients with adenomyosis uteri.