کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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3939696 | 1253567 | 2010 | 4 صفحه PDF | دانلود رایگان |
ObjectiveTo assess the cellular and molecular mechanisms of postoperative adhesion development in a rodent model.DesignProspective randomized controlled study.SettingResearch laboratory.PatientsThirty sexually mature female Sprague-Dawley rats.InterventionsCecal abrasion.Main outcome measure(s)At 0, 2, 4, 8, 16, 24, 48, 72, 96, 168, 336, and 504 hours after cecal abrasion, one to three rats were sacrificed (n = 26). Four nonabraded rats served as controls. Peritoneal adhesion status was evaluated and tissue was collected for histologic and immunohistochemical investigation.ResultsPostoperative tissue attachments were identified as early as 2 hours after cecal abrasion. Significant local edema and vessel congestion appeared within 2 hours, and cellular proliferation was observed at 24 hours; angiogenesis and tissue proliferation remained present at 2 weeks. β1 integrin was highly expressed early and was thereafter decreased. Cellular fibronectin was not detectable until 1 week after cecal abrasion.ConclusionsPostoperative adhesions are initiated as rapidly as 2 hours after surgical intervention in this rodent model.
Journal: Fertility and Sterility - Volume 93, Issue 8, 15 May 2010, Pages 2734–2737