کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3939946 1253574 2007 14 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Role for tumor necrosis factor alpha (TNF-α) and interleukin 1-beta (IL-1β) determination in seminal plasma during infertility investigation
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی زنان، زایمان و بهداشت زنان
پیش نمایش صفحه اول مقاله
Role for tumor necrosis factor alpha (TNF-α) and interleukin 1-beta (IL-1β) determination in seminal plasma during infertility investigation
چکیده انگلیسی

ObjectiveTo evaluate the clinical relevance of tumor necrosis factor alpha (TNF-α) and interleukin 1-beta (IL-1β) determination in seminal plasma during infertility investigation.DesignProspective study.SettingOutpatient infertility clinic of a university-based hospital.Patient(s)Randomly chosen asymptomatic males (n = 148) from subfertile couples.Intervention(s)None.Main Outcome Measure(s)Determination of TNF-α and IL-1β in seminal plasma (SP) by enzyme-linked immunosorbent assay (ELISA). In aliquots of the same ejaculates: 1) evaluation of semen quality with sperm analysis and sperm function testing; 2) determination of antisperm antibodies (ASA) of the immunoglobulin (Ig) G and IgA class; 3) microbial screening; and 4) immunocytochemical round cell differentiation to determine leukocyte counts and ratios. Medical history, clinical examination, and determination of subsequent fertility (after control for female infertility factors).Result(s)The concentrations of TNF-α and IL-1β in SP correlated significantly (r = 0.65; P<.0001), and these parameters were significantly related to the leukocyte ratio (%LC) of the seminal round cells (r = 0.36; P<.001) and the leukocyte counts per ejaculate (r = 0.34; P<.001). There was no relationship of TNF-α and IL-1β levels in SP with semen quality or parameters of sperm functional capacity, and there was no association with local ASA of the IgG or IgA class. The concentration of both cytokines was also not related to the outcome of the microbial screening and did not affect subsequent fertility. No correlation of TNF-α and IL-1β levels in SP with the concentration of C-reactive protein in same-day serum samples was found.Conclusion(s)The levels of TNF-α and IL-1β in seminal fluid correlate significantly with leukocyte counts and ratios in the same ejaculates, as indicators of silent male genital tract infection/inflammation. However, this is not related to semen cultures in asymptomatic individuals and not associated with clinically relevant parameters of semen quality, including sperm fertilizing capacity.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Fertility and Sterility - Volume 87, Issue 4, April 2007, Pages 810–823
نویسندگان
, , , , ,