کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3940158 1253580 2007 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Expression of syndecans, cell–cell interaction regulating heparan sulfate proteoglycans, within the human endometrium and their regulation throughout the menstrual cycle
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی زنان، زایمان و بهداشت زنان
پیش نمایش صفحه اول مقاله
Expression of syndecans, cell–cell interaction regulating heparan sulfate proteoglycans, within the human endometrium and their regulation throughout the menstrual cycle
چکیده انگلیسی

ObjectiveTo evaluate the expression of syndecan-1, -2, -3, and -4 in different phases of eutopic endometrium of normal cycling women.DesignProspective observational study.SettingUniversity-based research center for reproductive medicine.Patient(s)Twenty-nine healthy ovulatory volunteers.Intervention(s)mRNA and protein expression of syndecan-1 to -4 in human endometrium.Main Outcome Measure(s)Real-time polymerase chain reaction of syndecan members and further characterization of mRNA expression of syndecan-1 and -4 with multiprobe RNase protection assays of epithelial and stromal cells after purification with antibody-coated magnetic beads. For confirmation of results, protein expression and localization using immunohistochemistry for syndecan-1 and -4 was performed.Result(s)All syndecans were expressed within human endometrium. Syndecan-1 and -4 proved to be significantly upregulated in whole endometrium during the secretory phase (2.73-fold and 2.85-fold, respectively). Using multiprobe RNase protection assays, a significant upregulation of mRNA was noted in epithelial cells during the secretory phase for both syndecan-1 and -4 (7.46-fold and 2.52-fold, respectively) and confirmed by immunohistochemistry.Conclusion(s)Cycle-dependent expression of syndecan-1 and -4 suggests that these adhesion proteins are involved in the regulation of the cycling endometrium.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Fertility and Sterility - Volume 87, Issue 3, March 2007, Pages 657–663
نویسندگان
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