کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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3940415 | 1253587 | 2007 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: In vitro development of human oocytes after parthenogenetic activation or intracytoplasmic sperm injection In vitro development of human oocytes after parthenogenetic activation or intracytoplasmic sperm injection](/preview/png/3940415.png)
ObjectiveTo compare directly in vitro developmental competence between parthenogenetically activated and intracytoplasmic sperm injection (ICSI)-fertilized oocytes.DesignFor each patient, three metaphase II oocytes were randomized to the ICSI procedure, while n−3 were allocated to parthenogenetic activation.SettingUniversity hospital infertility unit.PatientsThirty-eight patients, aged 35.2 ± 3.3 years (mean ± SD) selected for ICSI.InterventionsAfter 1 hour from denudation, oocytes were either fertilized by ICSI (n = 114) or chemically activated (n = 104). Fertilized and activated oocytes were cultured for up to 3 and 5 days, respectively.Main Outcome MeasuresDevelopment rate, cell number, and morphological grade during culture.ResultsThe two groups showed no significant differences between rates of fertilization and parthenogenetic activation, development, and blastomere number on days 2 and 3 of culture. However, parthenotes showed a lower morphological grade, and a significantly lower proportion went on cleaving to day 3, when only activated rather than total numbers of oocytes were considered. On day 5 after activation, nine oocytes (8.6%) reached the blastocyst stage, representing 12.9% of parthenotes.ConclusionsSince most parameters examined in this study were similar between activated and fertilized oocytes, parthenogenetic activation may be a useful tool for the preclinical evaluation of experimental procedures.
Journal: Fertility and Sterility - Volume 87, Issue 1, January 2007, Pages 77–82