کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3941341 1253607 2009 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A novel approach for the selection of human sperm using annexin V-binding and flow cytometry
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی زنان، زایمان و بهداشت زنان
پیش نمایش صفحه اول مقاله
A novel approach for the selection of human sperm using annexin V-binding and flow cytometry
چکیده انگلیسی

ObjectiveTo develop a method whereby sperm with phosphatidylserine externalization can be separated from those without this feature. Because annexin V binds phosphatidylserine, this study is using this feature to select functional spermatozoa. In addition, the relationship between annexin V binding in human spermatozoa and normal sperm morphology according to strict criteria was to be assessed.DesignProspective study.SettingDepartment of Obstetrics and Gynaecology of Stellenbosch University at Tygerberg Academic Hospital, Tygerberg, South Africa.Patient(s)Semen from 14 healthy sperm donors. Exclusion criterion was the presence of less than 20 × 106/mL total motile spermatozoa in the original sample.Main Outcome Measure(s)Annexin V-negative sperm, annexin V-positive sperm, normal sperm morphology.Intervention(s)An aliquot of a semen sample after double density gradient centrifugation was incubated with annexin V fluorescein isothiocyanate conjugate (FITC). Cell fluorescence signals were determined using a FACScalibur flow cytometer equipped with a FACSSort fluidic sorting module. The sorting procedure delivered two sperm subpopulations: annexin V-negative and annexin V-positive. Morphology slides were made and the sperm morphology was assessed according to strict criteria.Result(s)There was a significant enrichment of annexin V-negative sperm as well as morphologically normal sperm in the annexin V-negative subgroup after separation with flow cytometry. The percentage of morphologically normal sperm increased from 8.3% in the control to 11.9% in the annexin V-negative fraction, whereas the percentage of annexin V-positive sperm decreased to 5.7%.Conclusion(s)The annexin V-negative sperm subpopulation had morphologically superior quality sperm compared to annexin V-positive sperm. It is important to select morphologically normal sperm during intracytoplasmic sperm injection (ICSI) as it may contribute to increased implantation and pregnancy rates (PR).

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Fertility and Sterility - Volume 91, Issue 4, April 2009, Pages 1285–1292
نویسندگان
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