کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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3941761 | 1253627 | 2008 | 10 صفحه PDF | دانلود رایگان |

ObjectiveTo evaluate the individual functionality of gonadotropin-stimulated preovulatory follicles, for understanding embryo failure in assisted reproductive technique cycles, in a sheep model.DesignObservational, model study.SettingPublic research unit.Animal(s)Fifteen adult Manchega ewes.Intervention(s)Synchronization of the estrous cycle with intravaginal progestagens and ovarian stimulation with FSH; evaluation of reproductive activity, plasma sampling, ovarian ultrasonography, and ovariectomies.Main Outcome Measure(s)Determination of estrus behavior, plasma and intrafollicular concentrations of E2 and inhibin A, number and size of ovarian follicles, and developmental competence of oocytes.Result(s)These results support the usefulness of serial measurements of plasma inhibin A for assessment of follicular growth during the FSH treatment, rather than of E2 assays commonly used. Functionality of FSH-stimulated preovulatory follicles is clearly disturbed, as confirmed by a negative correlation between follicular size and intrafollicular concentrations of inhibin A and E2 in preovulatory follicles after individual dissection; moreover, the ability of their oocytes to resume meiosis was diminished.Conclusion(s)Functionality of follicles in controlled ovarian stimulation (COS), and developmental competence of their oocytes, is disturbed by the high doses of gonadotropin supplied and finally determined by follicular sizes at starting FSH treatment.
Journal: Fertility and Sterility - Volume 89, Issue 5, Supplement, May 2008, Pages 1328–1337