کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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3942667 | 1254017 | 2015 | 8 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Glucose promotes cell proliferation, glucose uptake and invasion in endometrial cancer cells via AMPK/mTOR/S6 and MAPK signaling Glucose promotes cell proliferation, glucose uptake and invasion in endometrial cancer cells via AMPK/mTOR/S6 and MAPK signaling](/preview/png/3942667.png)
• High glucose promotes proliferation of endometrial cancer cells through AMPK/mTOR/S6 and MAPK signaling pathways.
• High glucose promotes cell cycle progression and induces the ability of adhesion and invasion in endometrial cancer cells.
• High glucose increases glucose uptake and glycolytic activity in endometrial cancer cells.
ObjectivesObesity and diabetes are well-known risk factors for the development of endometrial cancer. A high rate of aerobic glycolysis represents a key mechanism by which endometrial cancer cells consume glucose as its primary energy source. The up-regulated glycolytic pathway is a common therapeutic target whose inhibition has implications for anti-tumor activity in cancer cells. This study aimed to investigate the effect of various concentrations of glucose on cell proliferation in endometrial cancer.MethodsECC-1 and Ishikawa cells were treated with low glucose (1 mM), normal glucose (5 mM) and high glucose (25 mM), and cytotoxicity, apoptosis, cell cycle, adhesion/invasion, and changes of AMPK/mTOR/S6 and MAPK pathways were evaluated.ResultsOur results revealed that high glucose increased cell growth and clonogenicity in two endometrial cancer cell lines in a dose dependent manner. Low glucose induced the activity of cleaved caspase 3 and caused cell cycle G1 arrest. High glucose increased the ability of adhesion and invasion by decreasing E-cadherin and increasing Snail expression. In addition, high glucose increased glucose uptake and glycolytic activity through modulating the AMPK/mTOR/S6 and MAPK pathways.ConclusionsOur findings suggest that glucose stimulated cell proliferation through multiple complex signaling pathways. Targeting glucose metabolism may be a promising therapeutic strategy in the treatment of endometrial cancer.
Journal: Gynecologic Oncology - Volume 138, Issue 3, September 2015, Pages 668–675