کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3946364 1254336 2016 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Characterization of ovarian cancer cell lines as in vivo models for preclinical studies
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی زنان، زایمان و بهداشت زنان
پیش نمایش صفحه اول مقاله
Characterization of ovarian cancer cell lines as in vivo models for preclinical studies
چکیده انگلیسی


• Human cell line xenografts provide a valuable tool for studying cancer biology and response to treatments.
• Characterizing growth conditions in mice can speed translation of in vitro work to in vivo testing.
• The tumorigenicity of 17 human ovarian cancer cell lines is variable, and may reflect differing biology of each individual model.

ObjectiveThe value of cell lines for pre-clinical work lies in choosing those with similar characteristics. Selection of cell lines is typically based on patient history, histological subtype at diagnosis, mutation patterns, or signaling pathways. Although recent studies established consensus regarding molecular characteristics of ovarian cancer cell lines, data on in vivo tumorigenicity remains only sporadically available, impeding translation of in vitro work to xenograft models.MethodsWe introduced 18 ovarian cancer cell lines into athymic nude mice through subcutaneous, intraperitoneal, and ovary intrabursal routes, and observed tumor development over 6 weeks. We also profiled cell line gene expression and identified differentially expressed gene sets based on their ability to form tumors in the subcutaneous or intraperitoneal locations. Representative cell lines were further subjected to proteomic analyses.ResultsOvarian cancer cell lines showed variable ability to grow in mice when implanted subcutaneous, intraperitoneal, or intrabursal. While some cell lines grew well in both SC and IP locations, others showed a strong propensity to grow in one location only. Gene expression profiles suggested that cell lines showing preference for IP growth had gene expression patterns more similar to primary tumors.ConclusionsWe report the tumorigenicity of 17 human ovarian cancer cell lines and one mouse cell line in three distinct anatomical locations, and associated gene networks. Growth patterns and histopathology, linked to molecular characteristics, provide a valuable resource to the research community, and better guide the choice of cell lines for in vitro studies to translate efficiently into xenograft testing.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gynecologic Oncology - Volume 142, Issue 2, August 2016, Pages 332–340
نویسندگان
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