کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3963904 1600715 2011 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
In vitro human immunodeficiency virus and sperm cell interaction mediated by the mannose receptor
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ایمونولوژی
پیش نمایش صفحه اول مقاله
In vitro human immunodeficiency virus and sperm cell interaction mediated by the mannose receptor
چکیده انگلیسی

Leukocytes are considered to be the main source of HIV-1 infection in semen. However, HIV-1 interaction with spermatozoa has also been demonstrated, suggesting that both spermatozoa and leukocytes might play a role during sexual transmission of HIV-1. The purpose of the present study was to evaluate if HIV-1 particles interact with sperm cells through the mannose receptor (MR), and then to determine the ability of “infected” sperm cells to transmit the virus to susceptible targets. The expression of classical HIV-1 receptor and co-receptors and the MR by sperm cells was determined by flow cytometry; the interaction in vitro between sperm and HIV-1 was evaluated by fluorescence microscopy. Additionally, the in vitro interaction of sperm cells and HIV-1 was determined detecting viral nucleic acids by PCR. d-Mannose was used to block HIV-1–sperm cell interaction. Sperm cells preincubated with HIV-1 particles and activated mononuclear cells were co-cultured to determine viral transmission. The presence of viral RNA was detected in 28% of the samples in which sperm cells were preincubated with HIV-1 particles. Mannose was able to block interaction in 75% of the cases. Finally, we demonstrated that “infected” sperm cells were able to transmit the HIV-1 infection to susceptible targets. In conclusion, these results indicate that the MR is involved in sperm cell–HIV-1 interaction. Our results also suggest that sperm cells could be an important source of infection.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Reproductive Immunology - Volume 92, Issues 1–2, December 2011, Pages 1–7
نویسندگان
, , , , ,