کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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3964046 | 1600722 | 2007 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Toll-like receptor 3 agonist induces impairment of uterine vascular remodeling and fetal losses in CBA × DBA/2 mice Toll-like receptor 3 agonist induces impairment of uterine vascular remodeling and fetal losses in CBA × DBA/2 mice](/preview/png/3964046.png)
The objective of this study was to examine the expression of Toll-like receptor (TLR) 3 at the maternal–fetal interface and determine whether exposure to TLR3 agonist would induce an innate immune response and trigger pregnancy loss. To address this, abortion-prone male DBA/2J mated-CBA/J female mice were given polyinosinic–polycytidylic acid (poly I:C; 10 μg/g body weight, i.p.) or PBS at gestation day (gd) 6.5. All implantation sites appeared viable at gd 7.5 when endometrium was dissected for immunohistological examination. It was noted that poly I:C treatment increased fetal losses to 40.2 ± 1.7% at midgestation stage compared with control animals (11.0 ± 3.0%). It was observed also that the ratio of vessel to lumen area significantly increased at gd 10.5 and gd 12.5 after poly I:C treatment, indicating that the spiral artery (SA) modification was impaired. Meanwhile, 24 h after poly I:C injection, expression of TLR3 was markedly elevated within decidua basalis (DB), and endometrial TNF-α increased 2.7-fold but IFN-γ remained unchanged in homogenized endometrium. These results suggest that enhanced TNF-α expression in endometrial stroma may play a critical role in inflammatory factor production and impairment of uterine spiral artery remodeling in the pregnancy failure of CBA × DBA/2 mating.
Journal: Journal of Reproductive Immunology - Volume 74, Issues 1–2, June 2007, Pages 61–67