کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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3971542 | 1256769 | 2010 | 4 صفحه PDF | دانلود رایگان |
The objective was to report two singleton live births after transfer of cryopreserved–thawed day-3 embryos resulting from an unstimulated in-vitro oocyte maturation (IVM) cycle. A 29-year-old female patient with polycystic ovaries (PCO) underwent an unstimulated IVM cycle. A total of 43 prophase-I oocytes were retrieved; 21 oocytes achieved in-vitro maturation to the metaphase-II stage at 36 h post-retrieval and 18 oocytes were fertilized (two pronuclei) after intracytoplasmic sperm injection. Two embryos were transferred in the fresh cycle (no pregnancy) and 15 day-3 embryos (post-oocyte microinjection) were cryopreserved. Subsequently, the patient became pregnant after each of two embryo transfer cycles from cryopreserved–thawed embryos (three and two embryos transferred respectively), with delivery of a single, term, healthy baby after each transfer. It is concluded that healthy live births were documented in a PCO patient undergoing unstimulated IVM followed by transfer of day-3 cryopreserved (slow-freeze)–thawed embryos, adding these methodologies to the armamentarium of assisted reproductive technologies.
Journal: Reproductive BioMedicine Online - Volume 20, Issue 3, March 2010, Pages 387–390