کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4011469 1261148 2011 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Specific inhibition of Candida albicans growth in vitro by antibodies from experimental Candida keratitis mice
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ایمونولوژی و میکروب شناسی (عمومی)
پیش نمایش صفحه اول مقاله
Specific inhibition of Candida albicans growth in vitro by antibodies from experimental Candida keratitis mice
چکیده انگلیسی

To investigate the role of humoral immunity in the response to experimental keratitis, Balb/c mice were primed by one of three protocols: i) intranasal inhalation of live Candida spores; ii) subcutaneous injection of heat-inactivated spores; or iii) induction and healing of primary CaK. Experimental murine CaK was then induced in the three groups of primed mice and one group of unprimed mice by intrastromal injection of live Candida albicans spores. Totally 30 mice were included in each group. Sera collected after CaK induction were subjected to serial dilution and their effects on fungal growth and survival were tested as an assay for fungicidal activity in vitro. Corneas removed at various stages of disease were examined histologically, and fungal loads were determined using quantitative real-time PCR. Compared to corneas from mice with primary CaK, all corneas from CaK mice that had been previously primed exhibited milder histological disruptions that were faster to resolve, contained higher immunoglobulin and IFNγ titers, and had lower pathogen load (P < 0.05). Infiltration of pro-Inflammatory cells, which comprised mainly leukocytes other than lymphocytes, also initiated earlier in the primed mice compared to the controls (at day 3 versus day 7 respectively), and this should be due to differential production of cytokines. Sera from primed CaK mice exhibited stronger fungicidal activity and this was relatively specific for the original pathogen. Based on these findings, we proposed that the humoral response elicited by CaK plays important role in host protection against secondary C. albicans infections, and this might be achieved by pathogen-specific inhibition of fungal survival and/or growth.


► Humoral response in mice to experimental Candia albicans keratitis was studied.
► Corneas of primed mice exhibited milder disruptions and had lower pathogen loads.
► Sera from primed CaK mice exhibited stronger fungicidal activity.
► Fungicidal activity of sera in CaK mice were specific for the original pathogen.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Eye Research - Volume 93, Issue 1, July 2011, Pages 50–58
نویسندگان
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