کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4012919 1261219 2007 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A reference dataset for circular dichroism spectroscopy tailored for the βγ-crystallin lens proteins
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ایمونولوژی و میکروب شناسی (عمومی)
پیش نمایش صفحه اول مقاله
A reference dataset for circular dichroism spectroscopy tailored for the βγ-crystallin lens proteins
چکیده انگلیسی
Circular dichroism (CD) spectroscopy is a powerful solution technique for the study of protein secondary structure. As hierarchical euclidean clustering analyses of high quality crystallin synchrotron radiation circular dichroism (SRCD) spectral data can be separated into structural groups based solely on spectral information, the technique can potentially be improved to more accurately determine secondary structures and monitor conformational changes in crystallins. Secondary structure estimates can be determined through use of reference datasets of circular dichroism spectra from proteins with determined crystal structures. As with any empirical method, the accuracies of the analyses are dependent upon how closely the reference dataset characteristics match those of the protein to be studied. To date, crystallin proteins have not been well analysed by CD because existing reference datasets do not contain good representations of their structural characteristics. This work describes a βγ-crystallin specific reference dataset, CRYST175, which was created solely for the study of βγ-crystallin secondary structures. Prediction accuracy was assessed for the new dataset using several deconvolution algorithms and it was found to substantially outperform existing more general reference datasets.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Eye Research - Volume 84, Issue 5, May 2007, Pages 1001-1008
نویسندگان
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