کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4036064 1263569 2005 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Depolarizing effect of GABA in rod bipolar cells of the mouse retina
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی سیستم های حسی
پیش نمایش صفحه اول مقاله
Depolarizing effect of GABA in rod bipolar cells of the mouse retina
چکیده انگلیسی

Gamma-amino butyric acid (GABA) has been characterized as inhibitory neurotransmitter through chloride mediated channels in the adult nervous system. However, using gramicidin perforated patch-clamp recordings from rod bipolar cells dissociated from retinas of adult mice, we find that GABA is capable of inducing cell depolarization. Currents mediated by GABAA and GABAC receptors were further isolated by the use of GABA receptor specific blockers. In rod bipolar cells dissociated from the mouse retina, activation of GABAA receptors located at the cell dendrites induces ionic currents which show a reversal potential of −33 mV. However, local activation of GABAC receptors located at the axon terminal induces ionic currents with a reversal potential of −60 mV. According to Nernst equation, the dendrites of rod bipolar cells of the mouse retina would have a high intracellular chloride concentration ([Cl−]i) and there must be an intracellular gradient in [Cl−]i, being the [Cl−]i more elevated in the dendrites than in the axon terminal. The depolarizing effect of GABA at the dendrites of rod bipolar cells may contribute to the lateral interaction in the mammalian retina, thereby enhancing visual discrimination of stimuli input.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Vision Research - Volume 45, Issue 20, September 2005, Pages 2659–2667
نویسندگان
, , ,