کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4036344 1603246 2006 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Analysis of PDE6 function using chimeric PDE5/6 catalytic domains
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی سیستم های حسی
پیش نمایش صفحه اول مقاله
Analysis of PDE6 function using chimeric PDE5/6 catalytic domains
چکیده انگلیسی

cGMP-phosphodiesterases of the PDE6 family are expressed in retinal photoreceptor cells, where they mediate the phototransduction cascade. A system for expression of PDE6 in vitro is lacking, thus straining progress in understanding the structure–function relationships of the photoreceptor enzyme. Here, we report generation and characterization of bacterially expressed chimeric PDE5/6 catalytic domains which are highly soluble, catalytically active, and sensitive to inhibition by the PDE6 Pγ subunit. Two flexible PDE6 loops, H and M, impart chimeric PDE5/6 catalytic domains with PDE6-like properties. The replacement of the PDE6 H-loop into the PDE5 catalytic domain increases the catalytic rate and the Km value for cGMP hydrolysis, whereas the substitution of the M-loop produces catalytic PDE domains responsive to Pγ. Multiple PDE6 segments preventing functional expression of the catalytic domain are identified, supporting the requirement for specialized photoreceptor chaperones to assist PDE6 folding in vivo.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Vision Research - Volume 46, Issues 6–7, March 2006, Pages 860–868
نویسندگان
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