کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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4159435 | 1273826 | 2008 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Protective effect of erythropoietin pretreatment in testicular ischemia-reperfusion injury in rats Protective effect of erythropoietin pretreatment in testicular ischemia-reperfusion injury in rats](/preview/png/4159435.png)
Background/PurposeThis study was designed to investigate the effects of recombinant erythropoietin (EPO), a hormone widely used for treatment of uremic anemia, in rats subjected to testicular ischemia and reperfusion (I/R).MethodsThirty-five male rats were divided into the following: control, sham operated, ischemia (I), I/R, and I/R + EPO groups. In the I group, 2 hours of left unilateral testicular torsion were performed, and in the I/R and I/R + EPO groups, an additional 2 hours of testicular detorsions were performed. The I/R + EPO group was pretreated intraperitoneally with EPO (500 IU/kg) before reperfusion. Testicular tissue samples were examined for biochemical and histopathologic parameters. Apoptotic cells in all testes were detected by terminal deoxynucleotidyl transferase–mediated dUTP nick end-labeling technique and caspase 3 immunohistochemistry.ResultsAt histopathologic examination, ischemic changes in primary spermatocytes were noted in all torted testes. Cellular damage and apoptosis were more severe in ischemic groups than the EPO-pretreated group. There were statistically significant differences in tissue biochemical parameters in the I and I/R groups compared with the I/R + EPO group.ConclusionsThe results of the present study suggest that EPO exerts protective effects against I/R injury via the modulation of free radical scavenger's activities, which decreases lipid peroxidation levels and attenuation of apoptosis.
Journal: Journal of Pediatric Surgery - Volume 43, Issue 4, April 2008, Pages 722–728